Show simple item record

dc.contributor.authorLawrence, Janice
dc.contributor.authorTöpper, Joachim Paul
dc.contributor.authorPetelenz-Kurdziel, Elzbieta Anna
dc.contributor.authorBratbak, Gunnar
dc.contributor.authorLarsen, Aud
dc.contributor.authorThompson, Eric
dc.contributor.authorTroedsson, Christofer
dc.contributor.authorRay, Jessica Louise
dc.date.accessioned2017-11-10T10:06:49Z
dc.date.available2017-11-10T10:06:49Z
dc.date.created2017-10-23T15:16:47Z
dc.date.issued2017
dc.identifier.issn0024-3590
dc.identifier.urihttp://hdl.handle.net/11250/2465501
dc.description.abstractAppendicularians are planktonic marine tunicates with elaborate filter-feeding houses that can efficiently trap particles as small as 0.2 lm. While marine viruses are seldom considered outside their role in disease transmission, we conducted a controlled laboratory experiment to determine if the appendicularian Oikopleura dioica can trap and ingest the Emiliania huxleyi virus (EhV; 160–180 nm diameter). Removal and retention of EhV during 2.5 h and overnight incubations at 158C were measured using flow cytometry and quantitative polymerase chain reaction specific for the mcp gene of EhV. The fate of retained EhV was tested by quantifying EhV DNA in three biological compartments: house-trapping, ingestion/digestion, and defecation. Clearance rates for EhV varied from approximately 2 mL ind21 d21 to 50 mL ind21 d21, with highest rates for 4–5 d-old animals. EhV particles were cleared by O. dioica at rates similar to those reported for larger food particles, with mean clearance rates in the 2.5 h incubations ranging from approximately 2 mL ind21 d21 to 50 mL ind21 d21. This demonstrates efficient virus removal by O. dioica and a previously overlooked link between the microbial loop and the classical marine food web. EhV DNA was readily detectable above background levels in O. dioica houses, gut contents, and faecal pellets, suggesting that appendicularian houses and faecal pellets may contribute to the dispersal of viruses. Furthermore, clearance of EhV and presumably other viruses by O. dioica may be a significant sink for viruses and thus an important factor in regulating the population dynamics of viruses and their hosts.nb_NO
dc.language.isoengnb_NO
dc.titleViruses on the menu: The appendicularian Oikopleura dioica efficiently removes viruses from seawaternb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionacceptedVersionnb_NO
dc.subject.nsiVDP::Zoologiske og botaniske fag: 480nb_NO
dc.subject.nsiVDP::Zoology and botany: 480nb_NO
dc.source.journalLimnology and Oceanographynb_NO
dc.identifier.doi10.1002/lno.10734
dc.identifier.cristin1506918
dc.relation.projectNorges forskningsråd: 225956nb_NO
dc.relation.projectAndre: NSERCnb_NO
cristin.unitcode7511,8,0,0
cristin.unitnameBergen
cristin.ispublishedtrue
cristin.fulltextpostprint
cristin.qualitycode1


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record