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dc.contributor.authorAarnes, Siv Grethe
dc.contributor.authorFløystad, Ida
dc.contributor.authorSchregel, Julia
dc.contributor.authorVindstad, Ole Petter L.
dc.contributor.authorJepsen, Jane Uhd
dc.contributor.authorEiken, Hans Geir
dc.contributor.authorIms, Rolf Anker
dc.contributor.authorHagen, Snorre
dc.identifier.citationInternational Journal of Molecular Sciences 2015, 16:22541-22554nb_NO
dc.description.abstractThe autumnal moth (Epirrita autumnata) is a cyclically outbreaking forest Lepidoptera with circumpolar distribution and substantial impact on Northern ecosystems. We have isolated 21 microsatellites from the species to facilitate population genetic studies of population cycles, outbreaks, and crashes. First, PCR primers and PCR conditions were developed to amplify 19 trinucleotide loci and two tetranucleotide loci in six multiplex PCR approaches and then analyzed for species specificity, sensitivity and precision. Twelve of the loci showed simple tandem repeat array structures while nine loci showed imperfect repeat structures, and repeat numbers varied in our material between six and 15. The application in population genetics for all the 21 microsatellites were further validated in 48 autumnal moths sampled from Northern Norway, and allelic variation was detected in 19 loci. The detected numbers of alleles per locus ranged from two to 13, and the observed and expected heterozygosities varied from 0.04 to 0.69 and 0.04 to 0.79, respectively. Evidence for linkage disequilibrium was found for six loci as well as indication of one null allele. We find that these novel microsatellites and their multiplex-PCR assays are suitable for further research on fine- and large-scale population-genetic studies of Epirrita autumnata. tri- and tetranucleotide microsatellites; multiplex PCR; Lepidoptera; population geneticsnb_NO
dc.rightsNavngivelse-Ikkekommersiell 3.0 Norge*
dc.subjecttri- and tetranucleotide microsatellitesnb_NO
dc.subjectmultiplex PCRnb_NO
dc.subjectpopulation geneticsnb_NO
dc.titleIdentification and evaluation of 21 novel microsatellite markers from the autumnal moth (Epirrita autumnata) (Lepidoptera: Geometridae)nb_NO
dc.typePeer reviewednb_NO
dc.typeJournal article
dc.subject.nsiVDP::Mathematics and natural science: 400::Zoology and botany: 480nb_NO
dc.source.journalInternational Journal of Molecular Sciencesnb_NO
dc.relation.projectAndre: Norwegian Institute for Bioeconomy Research (NIBIO)nb_NO

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Navngivelse-Ikkekommersiell 3.0 Norge
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