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dc.contributor.authorAbdullah Al, Mamun
dc.contributor.authorChen, Huihuang
dc.contributor.authorYan, Xue
dc.contributor.authorRen, Kevin
dc.contributor.authorXue, Yuanyuan
dc.contributor.authorShimeta, Jeff
dc.contributor.authorMajaneva, Markus
dc.contributor.authorWilkinson, David M.
dc.contributor.authorYang, Jun
dc.date.accessioned2024-01-09T12:19:09Z
dc.date.available2024-01-09T12:19:09Z
dc.date.created2023-12-11T12:29:20Z
dc.date.issued2023
dc.identifier.issn1470-160X
dc.identifier.urihttps://hdl.handle.net/11250/3110610
dc.description.abstractHigh-throughput sequencing has revolutionized the DNA sequence-based study of microbial community and diversity on an unprecedented scale. Relic DNA is widespread in the natural aquatic environment; however, the extent to which such DNA can bias the sequence-based analysis of living microeukaryotic communities is unclear. Here, we conducted a 30-day field-control experiment investigating biofilm succession of microeukaryotic communities on glass slides and polyurethane foam units (PFUs) in a subtropical urban reservoir, using DNAbased approach. The propidium monoazide (PMA) dye was used to distinguish living and relic DNA sequences. Our results showed that microeukaryotic community succession in biofilms was time and substratum dependent, which was correlated with changing environmental conditions, nutrients and microalgae. Most importantly, relic DNA has a significant influence on the estimation of beta diversity and turnover in community composition, importantly its presence masked the successional patterns of different microbial taxa. We found strong deterministic processes dominating microeukaryotic succession on glass slides and PFUs biofilms. Depending on substrate and the presence and absence of relic DNA sequences, contrasting community assembly mechanisms were observed. In the presence of relic DNA, our results showed that 0.53–3.15 % of dispersal limitation, and 13.16 % of homogeneous selection processes were overestimated, while 1.06–3.68 % of homogenizing dispersal, 2.11–10.53 % of undominated and 2.10–10.52 % of heterogeneous selection processes were underestimated. This study provides significant insights into the value of removing relic DNA when designing DNA-based studies to characterize microeukaryotic communities and raise questions about some ecological interpretations in molecular microbial ecology which have not accounted for the effects of relic DNA.en_US
dc.language.isoengen_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.no*
dc.subjectPropidium monoazide (PMA)en_US
dc.subjectMicroeukaryotesen_US
dc.subjectEnvironmental DNAen_US
dc.subjectMicrobial communityen_US
dc.subjectSubtropical reservoiren_US
dc.titleRelic DNA confounds the results of DNA-based study on microeukaryotic succession in aquatic biofilmsen_US
dc.title.alternativeRelic DNA confounds the results of DNA-based study on microeukaryotic succession in aquatic biofilmsen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.rights.holder© 2023 The Authorsen_US
dc.subject.nsiVDP::Økologi: 488en_US
dc.subject.nsiVDP::Ecology: 488en_US
dc.source.volume158en_US
dc.source.journalEcological Indicatorsen_US
dc.identifier.doi10.1016/j.ecolind.2023.111350
dc.identifier.cristin2211702
dc.relation.projectAndre: National Natural Sci. Foundation of China:2251306 & 32100331en_US
dc.relation.projectAndre: Chinese Academy of Sciencesen_US
dc.relation.projectAndre: Natural Science Foundation of Fujian Province of Chinaen_US
dc.relation.projectAndre: CAS-TWAS President Fellowship (2019A8018137001)en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal
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