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dc.contributor.authorEkblom, Robert
dc.contributor.authorBrechlin, Birte
dc.contributor.authorPersson, Jens
dc.contributor.authorSmeds, Linnea
dc.contributor.authorJohansson, Malin
dc.contributor.authorMagnusson, Jessica
dc.contributor.authorFlagstad, Øystein
dc.contributor.authorEllegren, Hans
dc.coverage.spatialScandinavia, Skandinaviaen_US
dc.identifier.citationConservation Biology. 2018, 32 (6), 1301-1312.en_US
dc.description.abstractGenetic approaches have proved valuable to the study and conservation of endangered populations, especially for monitoring programs, and there is potential for further developments in this direction by extending analyses to the genomic level. We assembled the genome of the wolverine (Gulo gulo), a mustelid that in Scandinavia has recently recovered from a significant population decline, and obtained a 2.42 Gb draft sequence representing >85% of the genome and including >21,000 protein-coding genes. We then performed whole-genome resequencing of 10 Scandinavian wolverines for population genomic and demographic analyses. Genetic diversity was among the lowest detected in a red-listed population (mean genome-wide nucleotide diversity of 0.05%). Results of the demographic analyses indicated a long-term decline of the effective population size (Ne) from 10,000 well before the last glaciation to <500 after this period. Current Ne appeared even lower. The genome-wide FIS level was 0.089 (possibly signaling inbreeding), but this effect was not observed when analyzing a set of highly variable SNP markers, illustrating that such markers can give a biased picture of the overall character of genetic diversity. We found significant population structure, which has implications for population connectivity and conservation. We used an integratedmicrofluidic circuit chip technology to develop an SNP-array consisting of 96 highly informativemarkers that, together with a multiplex pre-amplification step, was successfully applied to low-quality DNA from scat samples. Our findings will inform management, conservation, and genetic monitoring of wolverines and serve as a genomic roadmap that can be applied to other endangered species. The approach used here can be generally utilized in other systems, but we acknowledge the trade-off between investing in genomic resources and direct conservation actions. genome assembly, non-invasive sampling, population genetics, single nucleotide polymorphismsen_US
dc.description.abstractSecuenciaci´on de Genomas y Gen´omica de la Conservaci´on para la Poblaci´on Escandinava de Glotonesen_US
dc.rightsNavngivelse-Ikkekommersiell 4.0 Internasjonal*
dc.subjectgenome assemblyen_US
dc.subjectnon-invasive samplingen_US
dc.subjectpopulation geneticsen_US
dc.subjectsingle nucleotide polymorphismsen_US
dc.titleGenome sequencing and conservation genomics in the Scandinavian wolverine populationen_US
dc.title.alternativeSecuenciaci´on de Genomas y Gen´omica de la Conservaci´on para la Poblaci´on Escandinava de Glotonesen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.rights.holder© 2018 The Authorsen_US
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474en_US
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480en_US
dc.source.journalConservation Biologyen_US
dc.relation.projectAndre: Norwegian Directorate for nature managementen_US
dc.relation.projectAndre: wedish Nature Protection Agencyen_US
cristin.unitnameAvdeling for terrestrisk økologi

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Navngivelse-Ikkekommersiell 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse-Ikkekommersiell 4.0 Internasjonal